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Nycomed optiprep density gradient
Optiprep Density Gradient, supplied by Nycomed, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optiprep+density+gradient/optiprep+gradient/pmc12109429-115-32-28
Average 90 stars, based on 1 article reviews
optiprep density gradient - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Isolation:

Article Title: Oral warfarin affects some aspects of systemic immunomodulation with topical dinitrochlorobenzene (DNCB) in rats.
Article Snippet: Purpose: The efficacy of topical dinitrochlorobenzene (DNCB) in treatment of some skin dermatoses is based both on local and systemic effects.. It is not known, however, whether it can be applied to patients receiving some other therapy associated with systemic immunomodulation.. The aim of the present paper using a rat model, was to examine whether oral warfarin (WF) intake, as shown by others and by us, had an immunomodulatory potential to interfere with effects of topical DNCB as systemic immunotherapy.

Article Title: Strain differences in toxicity of vitamin K antagonist warfarin in rats
Article Snippet: Hematoxylin and eosin (H&E)-stained histology slides were subsequently analyzed in a blinded manner by experienced pathologist using a Coolscope digital light microscope (Nikon Co., Tokyo, Japan). .. Peripheral blood granulocyte and mononuclear cell isolation Peripheral blood leukocytes were isolated from the heparinized blood by dextran sedimentation and centrifugation (700 g, 20 min at room temperature) on an OptiPrep (Nycomed AS, Norway) density gradient. ..

Article Title: Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats.
Article Snippet: Warfarin affects mainly vitamin K dependent (VKD) processes, but the effects on some non-VKD-related activities such as tumor growth inhibition and mononuclear cell-mediated immune reactions were shown as well.. In this study, the effect of subchronic (30 days) oral warfarin (0.35 mg/l and 3.5 mg/l) intake on peripheral blood granulocytes in rats was investigated.. Increase in prothrombin and partial thromboplastin time at high warfarin dose reflected its basic activity.

Article Title: Differential Proneness to Obesity in Two Rat Strains with Diverse Immune Responses
Article Snippet: Protein concentration was determined by Lowry assay [ ], using bovine serum albumin (Fraction V obtained from Sigma-Aldrich, St. Louis, MO, USA) as a reference. .. Peripheral blood mononuclear (PBMC) and polymorphonuclear cells (PMN) were isolated from the heparinized blood by dextran sedimentation and centrifugation (700× g , 20 min, 20 °C) on OptiPrep (Nycomed AS, Asker, Norway) density gradient. ..

Sedimentation:

Article Title: Oral warfarin affects some aspects of systemic immunomodulation with topical dinitrochlorobenzene (DNCB) in rats.
Article Snippet: Purpose: The efficacy of topical dinitrochlorobenzene (DNCB) in treatment of some skin dermatoses is based both on local and systemic effects.. It is not known, however, whether it can be applied to patients receiving some other therapy associated with systemic immunomodulation.. The aim of the present paper using a rat model, was to examine whether oral warfarin (WF) intake, as shown by others and by us, had an immunomodulatory potential to interfere with effects of topical DNCB as systemic immunotherapy.

Article Title: Strain differences in toxicity of vitamin K antagonist warfarin in rats
Article Snippet: Hematoxylin and eosin (H&E)-stained histology slides were subsequently analyzed in a blinded manner by experienced pathologist using a Coolscope digital light microscope (Nikon Co., Tokyo, Japan). .. Peripheral blood granulocyte and mononuclear cell isolation Peripheral blood leukocytes were isolated from the heparinized blood by dextran sedimentation and centrifugation (700 g, 20 min at room temperature) on an OptiPrep (Nycomed AS, Norway) density gradient. ..

Article Title: Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats.
Article Snippet: Warfarin affects mainly vitamin K dependent (VKD) processes, but the effects on some non-VKD-related activities such as tumor growth inhibition and mononuclear cell-mediated immune reactions were shown as well.. In this study, the effect of subchronic (30 days) oral warfarin (0.35 mg/l and 3.5 mg/l) intake on peripheral blood granulocytes in rats was investigated.. Increase in prothrombin and partial thromboplastin time at high warfarin dose reflected its basic activity.

Article Title: Differential Proneness to Obesity in Two Rat Strains with Diverse Immune Responses
Article Snippet: Protein concentration was determined by Lowry assay [ ], using bovine serum albumin (Fraction V obtained from Sigma-Aldrich, St. Louis, MO, USA) as a reference. .. Peripheral blood mononuclear (PBMC) and polymorphonuclear cells (PMN) were isolated from the heparinized blood by dextran sedimentation and centrifugation (700× g , 20 min, 20 °C) on OptiPrep (Nycomed AS, Asker, Norway) density gradient. ..

Centrifugation:

Article Title: Oral warfarin affects some aspects of systemic immunomodulation with topical dinitrochlorobenzene (DNCB) in rats.
Article Snippet: Purpose: The efficacy of topical dinitrochlorobenzene (DNCB) in treatment of some skin dermatoses is based both on local and systemic effects.. It is not known, however, whether it can be applied to patients receiving some other therapy associated with systemic immunomodulation.. The aim of the present paper using a rat model, was to examine whether oral warfarin (WF) intake, as shown by others and by us, had an immunomodulatory potential to interfere with effects of topical DNCB as systemic immunotherapy.

Article Title: Strain differences in toxicity of vitamin K antagonist warfarin in rats
Article Snippet: Hematoxylin and eosin (H&E)-stained histology slides were subsequently analyzed in a blinded manner by experienced pathologist using a Coolscope digital light microscope (Nikon Co., Tokyo, Japan). .. Peripheral blood granulocyte and mononuclear cell isolation Peripheral blood leukocytes were isolated from the heparinized blood by dextran sedimentation and centrifugation (700 g, 20 min at room temperature) on an OptiPrep (Nycomed AS, Norway) density gradient. ..

Article Title: Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats.
Article Snippet: Warfarin affects mainly vitamin K dependent (VKD) processes, but the effects on some non-VKD-related activities such as tumor growth inhibition and mononuclear cell-mediated immune reactions were shown as well.. In this study, the effect of subchronic (30 days) oral warfarin (0.35 mg/l and 3.5 mg/l) intake on peripheral blood granulocytes in rats was investigated.. Increase in prothrombin and partial thromboplastin time at high warfarin dose reflected its basic activity.

Article Title: Differential Proneness to Obesity in Two Rat Strains with Diverse Immune Responses
Article Snippet: Protein concentration was determined by Lowry assay [ ], using bovine serum albumin (Fraction V obtained from Sigma-Aldrich, St. Louis, MO, USA) as a reference. .. Peripheral blood mononuclear (PBMC) and polymorphonuclear cells (PMN) were isolated from the heparinized blood by dextran sedimentation and centrifugation (700× g , 20 min, 20 °C) on OptiPrep (Nycomed AS, Asker, Norway) density gradient. ..

Article Title: Selective Abrogation of Major Histocompatibility Complex Class II Expression on Extrahematopoietic Cells in Mice Lacking Promoter IV of the Class II Transactivator Gene
Article Snippet: To isolate splenic and thymic DCs, the organs were digested with collagenase D (Boehringer Mannheim) before preparation of single-cell suspensions as described . .. Low-density cell fractions enriched in DCs (∼ 10%) were then recovered by centrifugation over an OptiPrep TM (Nycomed) density gradient . ..

Clinical Proteomics:

Article Title: Oral warfarin affects some aspects of systemic immunomodulation with topical dinitrochlorobenzene (DNCB) in rats.
Article Snippet: Purpose: The efficacy of topical dinitrochlorobenzene (DNCB) in treatment of some skin dermatoses is based both on local and systemic effects.. It is not known, however, whether it can be applied to patients receiving some other therapy associated with systemic immunomodulation.. The aim of the present paper using a rat model, was to examine whether oral warfarin (WF) intake, as shown by others and by us, had an immunomodulatory potential to interfere with effects of topical DNCB as systemic immunotherapy.

other:

Article Title: A comparison of ovine monocyte-derived macrophage function following infection with Mycobacterium avium ssp. avium and Mycobacterium avium ssp. paratuberculosis.
Article Snippet: Mycobacterium avium ssp. paratuberculosis causes Johne’s disease in ruminants, whereas the antigenically and genetically similar subspecies Mycobacterium avium ssp. avium is less virulent.. In this study, we compared one strain of each subspecies for its ability to survive, induce cytokines, suppress MHC class I and II expression and induce apoptosis or necrosis in ovine monocyte-derived macrophages.. Both subspecies survived intracellularly and induced the secretion of IL-10.

Purification:

Article Title: Visualization of the intracellular behavior of HIV in living cells
Article Snippet: .. Purification of virus by gradient centrifugation was performed using an Optiprep (Nycomed) density gradient as described ( ). .. DiD (DiIC 18 ; Molecular Probes) labeling was achieved by addition of DiD (10 μM) after the initial wash, rinsing away unincorporated dye the next morning, and collecting supernatants as above.

Virus:

Article Title: Visualization of the intracellular behavior of HIV in living cells
Article Snippet: .. Purification of virus by gradient centrifugation was performed using an Optiprep (Nycomed) density gradient as described ( ). .. DiD (DiIC 18 ; Molecular Probes) labeling was achieved by addition of DiD (10 μM) after the initial wash, rinsing away unincorporated dye the next morning, and collecting supernatants as above.

Gradient Centrifugation:

Article Title: Visualization of the intracellular behavior of HIV in living cells
Article Snippet: .. Purification of virus by gradient centrifugation was performed using an Optiprep (Nycomed) density gradient as described ( ). .. DiD (DiIC 18 ; Molecular Probes) labeling was achieved by addition of DiD (10 μM) after the initial wash, rinsing away unincorporated dye the next morning, and collecting supernatants as above.

Cell Isolation:

Article Title: Strain differences in toxicity of vitamin K antagonist warfarin in rats
Article Snippet: Hematoxylin and eosin (H&E)-stained histology slides were subsequently analyzed in a blinded manner by experienced pathologist using a Coolscope digital light microscope (Nikon Co., Tokyo, Japan). .. Peripheral blood granulocyte and mononuclear cell isolation Peripheral blood leukocytes were isolated from the heparinized blood by dextran sedimentation and centrifugation (700 g, 20 min at room temperature) on an OptiPrep (Nycomed AS, Norway) density gradient. ..



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